Journal: Scientific Reports
Article Title: Renoprotective effects of ferric citrate in a mouse model of chronic kidney disease
doi: 10.1038/s41598-022-10842-4
Figure Lengend Snippet: Mineral metabolism parameters in Col4α3 knockout mice treated with or without 1% ferric citrate (FC) added to the diet. Parameters measured include: ( a ) serum phosphate, ( b ) urinary fractional excretion of phosphate, ( c ) bone Fgf23 mRNA, ( d ) marrow Fgf23 mRNA, ( e ) kidney Fgf23 mRNA, ( f ) liver Fgf23 mRNA, ( g ) spleen Fgf23 mRNA, ( h ) plasma C-terminal (total) FGF23, ( i ) plasma intact FGF23, ( j ) plasma percentage intact FGF23, ( k ) kidney Npt2a mRNA, ( l ) kidney Npt2c mRNA, ( m ) kidney Cyp27b1 mRNA, ( n ) kidney Cyp24a1 mRNA, ( o ) plasma parathyroid hormone, and ( p ) kidney Klotho mRNA. In figures ( e ), ( f ), ( g ), and ( l ), dotted lines correspond to quantitative real-time PCR cycle threshold values for blank samples. Data are presented as means and standard deviations. Two-tailed t-tests are used to compare groups, * p < 0.05.
Article Snippet: Commercially available enzyme-linked immunosorbent assays (ELISA) kits were used to measure urine albumin (Abcam, Cambridge, MA), serum erythropoietin (R&D Systems, Minneapolis, MN), serum amyloid A (R&D Systems), serum interleukin 1β (R&D Systems), serum interleukin 6 (R&D Systems), plasma parathyroid hormone (PTH) (Quidel, San Diego, CA), plasma C-terminal (total) fibroblast growth factor 23 (FGF23) (Quidel), and plasma intact FGF23 (Quidel).
Techniques: Knock-Out, Real-time Polymerase Chain Reaction, Two Tailed Test